Director:

Rudra Channappanavar, BVSc, PhD.  
Dr. Channappanavar is a viral immunologist with 15 years of experience studying immunology of infectious diseases. He has extensive expertise in flow cytometry/immunophenotyping, multiplex cytokine assays, ELISA, immunofluorescence, immunohistochemistry, and serological assays. 

Co-Director: 

Sunil Nivrutti More, BVSc & AH, MVSc, PhD, Diplomate ACVP
Dr. Sunil More is a board-certified veterinary anatomic pathologist specializing in respiratory pathology and host–pathogen interactions. His research focuses on viral respiratory infections and their bacterial complications, with particular emphasis on disease mechanisms and pathogenesis. He has extensive expertise in developing respiratory disease models and lesion characterization with quantitative lesion scoring, providing rigorous pathological endpoints for experimental studies.

Laboratory Manager:

Histotechnologist:

Service

Unit Charge

1.

Histopathologic evaluation

$120/hour

2.

Gross necropsy eval, tissue collection, and histopathology

$150/hour

3.

Tissue trimming only

$6/cassette

4.

Processing fixed tissue and paraffin embedding only

$8/block

5.

Unstained slides or scrolls from tissue or existing block 

$10/slide

6.

Trimming, processing and H&E or special stain slide prep (no interpretation)

$13/slide

7.

Special stains from paraffin embedded tissue

$8/slide

8.

Research IHC*

$50/slide

*Cost: may increase with optimization, labor, cost of primary antibody, purchase of antibody, etc. Adjusted as needed

9.

Recuts from paraffin embedded tissue**

$10/slide

**Cost: may increase with labor, depth required, attempts, etc. Adjusted as needed.

10.

Slide scanning

$5/slide

11.

Trimming, processing, and H&E slide prep (non-research, with interpretation)

$50/animal (Up to 4 slides)

12.

Cryostat (unassisted)

$15/hour

13.

Cryostat (assisted)

$45/hour

14.

Large slide box

$14/box

15.

Small slide box

$8/box

16.

Technical support including training, consultation, IHC/special stain optimization, etc.

$60/hour

Service

Unit Charge

FACS Aria with Operator

$60/hour

FACS Aria self-service (only well-trained personnel)

$30/hour

FACS Aria- Cell Sorting

$80/hour

Cytek  Aurora Evo Spectral flow cytometer (Self-service)

$30/hour

Cytek  Aurora Evo Spectral flow cytometer (Assisted service)

$60/hour

Flow Cytometry- Data Analysis

$30/hour

Bio-Plex Immunoassay

$45/plate

Bio-Plex Immunoassay- Data Analysis

$30/plate

NanoCellect WOLF cell sorter

$45/hour

Biotek Cytation 5 (Self-Service)

$15/hour

Biotek Cytation 5 (Assisted service)

$45/hour

Biotek Cytation 10 (Self-Service)

$30/hour

Biotek Cytation 10 (Assisted service)

$60/hour

Zeiss 900 LSM (Self-Service)

$30/hour

Zeiss 900 LSM (Assisted service)

$60/hour

Dispens Cell (Self-Service)

$30/hour

Dispens Cell (Assisted service)

$60/hour

The Aria has 4 lasers (violet, blue, yellow-green, red) with 13 fluorescence parameters for phenotyping. It is capable of aseptic, 4-way (4 cell types) sorts. The automated cell deposition unit (ACDU) allows sorting into multiwell plates and on microscope slides. The cytometer is housed in a Baker BioPROTECT IV biosafety cabinet to allow sorting of BSL2 infected cells or pathogens.
For assistance in designing a panel please contact Ms. Shannon Cowan or refer to this SITE.

Flow Cytometry Appointment Policy
Please cancel your flow cytometry appointments as soon as you are aware that you will not be able to attend. Cancellations made less than one hour before the scheduled time will incur a charge equivalent to one hour of instrument use. Failure to show up for a scheduled appointment will also result in a one-hour instrument charge. Please note that billing is based on the scheduled start time of your appointment, not the actual time of arrival.

Best Practices for Flow Cytometry
Best Practices for Flow Cytometry2

The Bio-Plex 200 system is a suspension array system which offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample. The instrument is suitable to run any kit that is Luminex 200 compatible. For additional details see this SITE.

This Cell Sorter allows for gentle analysis, sorting and plating of cells based on its microfluidic technology. By using sterile, disposable fluidics, system clean-up becomes extremely fast and cross contaminations are prevented. Therefore, the WOLF Cell Sorter is intended for use in single-cell genomics, antibody production, and cell line development.

The IX81 is Olympus' most advanced motorized microscope, providing high standards of observation, measurement, and functional operation. A V-shaped optical path, improved fluorescence illuminators and further expanded UIS optics provide excellent performance for research applications.

BioTek Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. For application details, please see this SITE.

The SpectraMax M2 is a dual monochromator, multi-detection microwell reader with dual mode cuvette port and 96 or 384 microwell reader capability. It utilizes UV-Vis absorbance, as well as fluorescence intensity (FI) with optical performance comparable to top-level dedicated fluorescence spectrometers or spectrophotometers. Use this reader for Endpoint, Kinetic, Spectral, Area Well Scans, DNA, RNA and protein quantification and purity; PicoGreen, NanoOrange and Bradford; ELISA and enzyme kinetics; drug lysis profiles; live or dead viability, cytotoxicity, Caspase-3 and protease assays; cAMP assays using the CatchPoint kit.

The SpectraMax Plus is a UV/Vis spectro-photometer/microwell reader with built-in cuvette port and microplate drawer. It can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. It can read 96 wells, each well has its own sample beam and reference detector. Applications include DNA Analysis, ELISA, Endpoint Analysis, Kinetics.

ELISA Plate Washer allows fast and easy washing of ELISA plates.

The Leica CM1850 is a high-precision cryostat microtome designed for sectioning frozen tissue specimens in a variety of biological and medical applications. The CM1850 is widely used in research and clinical settings for creating thin, high-quality tissue sections that are used in various types of microscopy, including light, fluorescence, and electron microscopy.

The ASP300 performs a series of steps to ensure that tissue samples are dehydrated, cleared and infiltrated with paraffin to allow for precise embedding and cutting. Tissues can then be used for a multitude of staining procedures.

The microtome and water bath will enable users to cut sections and mount on microscope slides. These are available for users on Do-It-Yourself basis and a small fee will be charged to equipment use. IPC staff will provide training to use this equipment.

The IPC Histology Lab can trim, process, embed, cut, and stain samples and slides using the following equipment on a fee-for-service basis. Equipment is managed, maintained and operated by IPC personnel.

Samples will be embedded on a Leica EG1160 Embedding Station.