About

Pilot project investigators, OCRID faculty, and other Oklahoma investigators may have limited animal research experience and limited biosafety level training in in vivo preclinical experimentation of respiratory and infectious diseases. In addition, these researchers will need in vivo validation of in vitro discoveries. As a result, consistent care, experimental procedures with pathogens in vivo, and animal husbandry are important for the success of the pilot projects, funding applications, and manuscript publication. Therefore, the objective of the Animal Models Core (AMC) is to ensure experimental consistency, compliance with animal welfare and pathogen safety regulations, and to provide training in animal model phenotyping and cutting-edge respiratory and infectious disease experiments.

OCRID Core Facilities Service Request Form

Please use the online form linked below to request a service from our core facility. Thank you!

Animal Models Core Service Request Form

OCRID Core Facilities User Satisfaction Survey

OCRID core facilities are committed to providing top quality services and technical expertise in respiratory and infectious disease for center investigators as well as non-center investigators. We encourage you to fill out our quick 10 question survey so your answers, comments and suggestions can help us improve our services. 

Core Facilities User Satisfaction Survey
 

• People

  Director

  Myron Hinsdale, Ph.D, CVM, OSU

  Associate Professor

  Department of Physiological Sciences

  Center for Veterinary Health Sciences

  Oklahoma State University-Stillwater

   

  Contact Information:

  E-mail: myron.hinsdale@okstate.edu

  Phone: (405) 744-8107
  Office: Rm 264, McElroy Hall

               Stillwater, OK 74078

  Biosketch: Dr. Hinsdale

   

  Co-Director

  Rudra Channappanavar, Ph.D,

  Assistant Professor 

  Veterinary Pathobiology

  College of Veterinary Medicine

  Oklahoma State University

   

  Contact Information:

  Email: rchanna@okstate.edu 

  Phone: 405-744-7224

  Office: 215

  Biosketch: Dr. Channappanavar

   

   

   

  ---

  Graduate Student

  Nabin Poudel 
  BVSc – Tribhuvan University, Nepal
  Thesis Advisor:  Dr. Myron Hinsdale
  Research Topic:  Extracellular matrix in cell proliferation

• Why is it important?

  Services & Equipment

  

  • Mouse colony management, genotyping, and husbandry
  • Animal model experiment design
  • Assistance with Vertebrate section of grant
  • Assistance with animal use protocol
  • Isolation of adipose tissue MSCs and characterization
  • Therapeutic intratracheal instillation of MSCs.
  • Viral and bacterial instillation for respiratory disease models
  • Respiratory physiology and mechanics analyses using SCIREQ flexiVent-3, Module 1
  • Blood analyzer for clinical chemistries and blood gases using i-STAT, Model i-STAT 1 IA-Abbot 
  • Real time continuous blood oxygenation analyses using MouseOx Plus, neonatal mice to adults.
  • Full anesthetic support with:
    • Vaporstick and anesthesia machine, precision vaporizer
    • Vinv-smit-0141  Rodent Induction chamber
    • Vinv-smit-0128  Rodent respiration circuit set
  • Xtreme (by Bruker) in vivo luminescent imaging with precise animal anatomic localization of mammalian and bacterial cells.

  If you have questions or would like to use any of these services please see the "Forms" tab or contact Dr. Myron Hinsdale at myron.hinsdale@okstate.edu with "AMC OCRID" in the subject line. 

• BSL 2 Services Fee Sheet

  PDF Version

   

  Animal Models Core: BSL2

  Service		Unit Charge	Service		Unit Charge
  1. GENOTYPING, ABSL-2 PPE, and OTHER CHARGES			3. EQUIPMENT USE/FEE (ABSL2)
  	ABSL-2 PPE exclusive use rate	$5/day		Pathogens or drug aerosolization - whole body nebulizer	$90/hour
  	Animal strain genotyping (PCR) per locus/phenotyping	$100/1-10 animals		Whole body plethysmography	$100/hour
  	Breeding, weaning, toeing, and line maintenance-basic husbandry	$72/hour		In Vivo Xtreme Whole Body Imaging	$90/hour
  2. MOUSE INFECTION, VIROLOGY, AND IMMUNOLOGY SERVICES (ABSL-2) (All assisted service)				Pulse oximetry	$90/hour
  	Mouse infection	$90/hour (10-15 mice)		Pathogen neutralization in cells or tissues (e.g., fixation)	$90/hour
  	Daily body weights and clinical assessment	$72/hour (10-15 mice)		Miltenyi MACS column-based separation of cells	$90/3 samples
  	Dissection and tissue harvesting	$72/hour		Miltenyi Biotec gentleMACS Octo tissue dissociator	$90(1-8 samples)/hour
  	Tissue preparation for specific processing (e.g., RNA and protein isolation)	$72/hour		Clinical chemistry analysis (e.g., iSTAT)	$22.50/sample
  	Tissue preparation for virus and bacteria titration	$72/hour		Piccolo Express analyses	$20/sample
  	Isolation of cells for downstream analyses by other cores (e.g., for FACS or IHC)	$72/hour		Anesthesia equipment usage (checked out w/O2&Isoflur.)	$40/day, $75/week
  	Animal euthanasia	$72/hour		Anesthesia equipment usage(checked out w/O2&Isoflur.)	$75/week

  The listed prices are for academic users. Active CoBRE pilot projects will be charged 25% of the fee schedule and non-academic users will be charged 1.496 of the fee schedule. b) Project-specific consumables and reagents will be provided by or additionally charged to the investigators. c) Un-assisted services charges for fully trained personnel on BSL-2 studies will be $30/hour.

• BSL 3 Services Fee Sheet

  PDF Version

   

  Animal Models Core: BSL3

  Service		Unit Charge	Service		Unit Charge
  1. ABSL-3 PPE			3. EQUIPMENT USE/FEE (ABSL3)
  	ABSL-3 PPE exclusive use rate	$10/day		Aerosolization of pathogens or drugs using whole body nebulizer	$60/hour
  2. MOUSE INFECTION, VIROLOGY, AND IMMUNOLOGY SERVICES (ABSL-3) (All assisted service)				Whole body plethysmography	$60/hour
  	Mouse infection	$60/hour (10-15 mice)		Pulse oximetry	$60/hour
  	Daily body weights and clinical assessment	$60/hour (10-15 mice)		Pathogen neutralization in cells or tissues (e.g., fixation)	$60/hour
  	Dissection and tissue harvesting	$60/hour		Miltenyi MACS column-based separation of cells	$60/hour
  	Tissue preparation for RNA and protein isolation	$60/hour		Clinical chemistry analysis (e.g., iSTAT)	$25/sample
  	Tissue preparation for virus and bacteria titration	$60/hour
  	Isolation of cells for downstream analyses by other cores (e.g., for FACS or IHC)	$60/hour
  	Animal euthanasia	$60/hour

  The listed prices are for academic users. Active CoBRE pilot projects will be charged 25% of the fee schedule and non-academic users will be charged 1.496 of the fee schedule. b) Project-specific consumables and reagents will be provided by or additionally charged to the investigators.