The specific aim of the Immunopathology Core Program (IPC) is to provide pathology and immunology support to COBRE and other Oklahoma Center for Respiratory and Infectious Diseases (OCRID) investigators. Services provided by the IPC include a broad range of pathology support including gross necropsy and tissue collection, tissue processing, sectioning of either frozen or paraffin-embedded tissues, routine and special stains, immunohistochemistry (IHC), immunofluorescence (IFC), and coordination of ancillary services (clinical pathology, bacteriology, virology, toxicology). Immune responses important in the development of disease are characterized by evaluation of cell-mediated immunity (ELISPOT, flow cytometry for intracellular IFNγ) and phenotypic identification of inflammatory cells within lesions (IFC, IHC, flow cytometry). The IPC will also coordinate with the Animal Models Core (AMC) and Molecular Biology Core (MBC) to assist investigators with humoral immunity (serology, ELISA) and quantitation of cytokine gene expression (real-time PCR) respectively. To achieve this aim, the IPC is staffed by three board-certified pathologists with experience in animal models and respiratory/infectious disease research and will recruit an experienced, extensively cross-trained technician. The IPC has the resources in place to serve as the foundation and a strong institutional commitment to ensure development of the IPC into a self-sustaining facility integral to the research infrastructure of OCRID.
The Immunopathology Core Program provides pathology support for animal modeling studies including: Study design, gross necropsy and evaluation, lesion scoring, gross lesion photography, tissue processing for histopathology, routine and special staining, histopathologic assessment, digital micrographs, histopathology lesions scoring, reporting, assistance with interpretation, manuscript and/or grant preparation.
The Immunopatholgoy Core Program also provides support for characterization of immune responses including flow cytometry, cell sorting and recovery, cytokine gene or protein expression, immunohistochemistry, immunofluorescence, ELISA.
The Immunopathology Core Laboratory is located on the 1st Floor, south wing of McElroy Hall (Room 171 McElroy). Contact Dr. Jerry Ritchey or Ms. Marie Montelongo (Laboratory Manager) for assistance.
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- BDFACSAria III- 4-lasers (blue, red, violet, yellow-green); high speed sorting capability;
sterile sort for retrieval of population, sort into tubes or plates; capability for
single cell retrieval.
- Preferred fluorochromes: FITC, PE, PerCP, PerCP Cy5.5, PE Cy7, APC, APC-Cy7, Pacific Blue/BV421, AmCyan/BV500, PE-Texas Red/mCherry, PE-Cy5
- FACSAria operation by technician or technician supervised only.
- BDFACSCalibur- 2 lasers, excellent analysis machine. No sorting capability.
- Preferred fluorochromes: FITC, PE, PerCP, APC (or any substitute within same excitation/emission parameters)
- FACSCalibur can be operated by user at reduced cost. Users must receive training (provided by Core lab).
- Shandon carousel tissue processor
- Leica robotic stainer
- Leica embedding station
- Leica microtome
- Heated water bath
- All processing and staining equipment located within large fume hood.
- Tissue trim station as well as cover slip station
- Core pathologists have their own light microscopes, digital micrograph capability
- Aperio slide scanner for digitized images
- Access to Core Electron Microscopy laboratory with Scanning, Transmission electron microscopes and Confocal microscope
Characterization of Immune Response
- Basic laboratory equipment
- 3-Thermocyclers (2 Eppendorf, 1 MJ Research)
- Biosafety cabinet
- Refrigerated microfuge
- Table-top refrigerated centrifuge
- ELISA plate washer
- CO2 incubators and equipment for cell culture
- Flow cytometery and cell sorting (above)
- Dedicated Laboratory computers/printers/IT support
- BDFACSAria III- 4-lasers (blue, red, violet, yellow-green); high speed sorting capability; sterile sort for retrieval of population, sort into tubes or plates; capability for single cell retrieval.