Immunopathology Core
About
The Immunopathology Core (IPC) provides state-of-the-art immunology and pathology services to COBRE, OCRID, OSU/Oklahoma, and external investigators. The IPC provides advanced immunology and pathology techniques, instrumentation, and expertise required for complex analyses. This core facility serves as a centralized resource offering technical services essential for advancing research primarily in respiratory and infectious diseases. In addition, the IPC collaborates with other OCRID cores, such as the Animal Models Core (AMC) and Molecular Biology Core (MBC) to provide streamlined services to users. The IPC is staffed with an expert immunologist and a board-certified veterinary pathologist each with extensive expertise in animal models and respiratory/infectious disease research. Additional support also includes an experienced core manager who is well trained in advanced immunology and pathology techniques and a histotechnologist to provide additional technical support and enhance service delivery.
COBRE funding in Phases I-II facilitated the establishment, expansion, and enhancement of IPC equipment, personnel, and services. The goals of the IPC during the current Phase III COBRE funding are focused on developing the core into a self-sustaining entity by providing state-of-the-art equipment use to OCRID, Oklahoma, and external investigators, including those from other veterinary colleges.
OCRID Core Facilities Service Request Form
Please use the online form linked below to request a service from our core facility. Thank you!
- People
Director:
Rudra Channappanavar, BVSc, PhD. Dr. Channappanavar is a viral immunologist with 15 years of experience
studying immunology of infectious diseases. He has extensive expertise in flow cytometry/immunophenotyping,
multiplex cytokine assays, ELISA, immunofluorescence, immunohistochemistry, and serological
assays. Co-Director:
Alexandra K Ford, DVM, DACVP. Dr. Ford is a board-certified veterinary anatomic pathologist. She has
extensive expertise in pathology of infectious and non-infectious diseases as well
as immunohistochemistry and other immunology and serology assays.Laboratory Manager:
Ms. Shannon Cowan, BS. Ms. Cowan is a skilled in multi parametric flow cytometry and multiplex cytokine
assays including prep, collection and analysis. She is a BD trained flow cytometry
expert and a cross trained expert in histotechnology and immunohistochemistry. Ms.
Cowan manages day-to-day IPC activities and is the primary contact for IPC related
questions.Histotechnologist:
Mr. Caden Miller. Caden is an undergraduate student working as a part-time histotechnician in IPC
for the last 3 years. -
Contact Information
The Immunopathology Core
171 McElroy Hall
Stillwater, 74078
Phone: 405-744-4749
Email: immunopathologycore@okstate.edu
Reach out to us! Our staff and directors are always eager to discuss how we can best facilitate your research needs.
- Price Information
Immunology Services
Service:
Unit Charge:
FACS Aria with Operator
$60/hour
FACS Aria self-service (only well-trained personnel)
$30/hour
FACS Aria- Cell Sorting
$75/hour
Flow Cytometry- Data Analysis $30/hour
Bio-Rad Bio-Plex Immunoassay
$45/plate
Bio-Rad Bio-Plex Immunoassay- Data Analysis
$30/hour
NanoCellect WOLF cell sorter
$45/hour
Olympus IX81 motorized fluorescence microscope (Self-Service)
$15/hour
Olympus IX81 motorized fluorescence microscope (Assisted service)
$45/hour
Biotek Cytation 5 (Self-Service)
$15/hour
Biotek Cytation 5 (Assisted service)
$45/hour
Histology Services
Service:
Unit Charge:
Gross necropsy eval including tissue collection $120/hour Histopathologic eval including H&E slides $120/hour Gross necropsy eval, tissue collection, and histopathologic evaluation $100/hour Tissue trimming only $6/cassette Processing fixed tissue and paraffin embedding only (no tissue trimming) $8/block Unstained slides from paraffin-embedded tissue $10/slide H&E slide preparation from paraffin-embedded tissue $10/slide Trimming, processing, and H&E slide preparation (no interpretation) $13/slide Processing, and H&E slide preparation (no trimming, no interpretation) $12/slide Fite’s acid-fast staining $8/slide Research decalcification $12/sample Serial unstained slides $7/slide Serial H&E stained slides $7/slide Research IHC
$50/slide
Recuts from paraffin embedded tissue
$10/slide
Large slide box
$14/box
Small slide box
$8/box
Slide scanning
$5/slide
Technical support (training, consult, IHC/special stain optimization etc.)
$60/hour
Trimming, processing, and H&E slide preparation (non-research, with interpretation)
$30/animal
Cryostat (unassisted/self-use)
$15/hour
- Equipment
FACSAria Special Order Research Product: 
The Aria has 4 lasers (violet, blue, yellow-green, red) with 13 fluorescence parameters for phenotyping. It is capable of aseptic, 4-way (4 cell types) sorts. The automated cell deposition unit (ACDU) allows sorting into multiwell plates and on microscope slides. The cytometer is housed in a Baker BioPROTECT IV biosafety cabinet to allow sorting of BSL2 infected cells or pathogens.
For assistance in designing a panel please contact Ms. Shannon Cowan or refer to this SITE.
Bio-Rad Bio-Plex 200 system: 
The Bio-Plex 200 system is a suspension array system which offers protein and nucleic
acid researchers a reliable multiplex assay solution that permits analysis of up to
100 biomolecules in a single sample. The instrument is suitable to run any kit that
is Luminex 200 compatible. For additional details see this SITE.NanoCellect WOLF Cell Sorter: 
This Cell Sorter allows for gentle analysis, sorting and plating of cells based on its microfluidic technology. By using sterile, disposable fluidics, system clean-up becomes extremely fast and cross contaminations are prevented. Therefore, the WOLF Cell Sorter is intended for use in single-cell genomics, antibody production, and cell line development.
Olympus IX81 motorized fluorescence microscope: 
The IX81 is Olympus' most advanced motorized microscope, providing high standards of observation, measurement, and functional operation. A V-shaped optical path, improved fluorescence illuminators and further expanded UIS optics provide excellent performance for research applications.
BioTek Cytation 5: 
5 combines automated microscopy and conventional microplate detection in a configurable,
upgradable platform. The microscopy module offers up to 60x magnification in fluorescence,
brightfield, high contrast brightfield, color brightfield, and phase contrast to address
many applications and workflows. For application details, please see this SITE.Molecular Devices SpectraMax M2: 
The SpectraMax M2 is a dual monochromator, multi-detection microwell reader with dual
mode cuvette port and 96 or 384 microwell reader capability. It utilizes UV-Vis absorbance,
as well as fluorescence intensity (FI) with optical performance comparable to top-level
dedicated fluorescence spectrometers or spectrophotometers. Use this reader for Endpoint,
Kinetic, Spectral, Area Well Scans, DNA, RNA and protein quantification and purity;
PicoGreen, NanoOrange and Bradford; ELISA and enzyme kinetics; drug lysis profiles;
live or dead viability, cytotoxicity, Caspase-3 and protease assays; cAMP assays using
the CatchPoint kit.Molecular Devices SpectraMax Plus: 
The SpectraMax Plus is a UV/Vis spectro-photometer/microwell reader with built-in cuvette port and microplate drawer. It can read any standard cuvette, micro cuvette, test tube and 96-well or UV-transparent microplate in the wavelength range of 190-1000 nm. It can read 96 wells, each well has its own sample beam and reference detector. Applications include DNA Analysis, ELISA, Endpoint Analysis, Kinetics.
ELISA Plate Washer: 
ELISA Plate Washer allows fast and easy washing of ELISA plates.Leica CM1850: 
The Leica CM1850 is a high-precision cryostat microtome designed for sectioning frozen tissue specimens in a variety of biological and medical applications. The CM1850 is widely used in research and clinical settings for creating thin, high-quality tissue sections that are used in various types of microscopy, including light, fluorescence, and electron microscopy.
Leica ASP300 Tissue Processor: 
The ASP300 performs a series of steps to ensure that tissue samples are dehydrated, cleared and infiltrated with paraffin to allow for precise embedding and cutting. Tissues can then be used for a multitude of staining procedures.
Shandon Microtome and water bath: The microtome and water bath will enable users to cut sections and mount on microscope slides. These are available for users on Do-It-Yourself basis and a small fee will be charged to equipment use. IPC staff will provide training to use this equipment.
The IPC Histology Lab can trim, process, embed, cut, and stain samples and slides using the following equipment on a fee-for-service basis. Equipment is managed, maintained and operated by IPC personnel.
Sakura Finetek Tissue-Tek VIP6 AI tissue processor: Sakura Finetek Tissue-Tek VIP6 AI tissue processor uses advanced infiltration designed to provide high-quality tissue processing results for all tissue types. 
Leica EG1160 Embedding Station: Samples will be embedded on a Leica EG1160 Embedding Station.
Leica HistoCore Biocut AS: 
Leica HistoCore Biocut AS used to cut sections for investigators.Sakura Finetek Tissue-Tek DRS 601 Slide Stainer 
Slides are stained using the Sakura Finetek Tissue-Tek DRS 601 Slide Stainer.
OCRID Core Facilities User Satisfaction Survey
OCRID Core facilities are committed to providing top quality services and technical expertise in respiratory and infectious disease for center and non-center investigators. We encourage all users to fill out a simple, ten question survey. Your responses, comments, and suggestions can help us improve our services.