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About

Molecular biology approaches are utilized to understand the molecular mechanisms of respiratory diseases caused by respiratory pathogens, including, not limited to influenza virus, SARS-CoV-2, RSV, Streptococcus pneumoniae and Pseudomonas aeruginosa. The overall objective of the Molecular Biology Core (MBC) is to provide centralized services and technical expertise in molecular biology for CoBRE project investigators, pilot project investigators, and other investigators. The specific aims of the MBC are to provide services and support for 1) functional studies using RNAi and CRISPR/Cas9, transgene overexpression, and mutagenesis/deletion, 2) genome-wide gene profiling using RNA sequencing, 3) molecular assays including reporter assays and real-time quantitative PCR, 4) reagents for respiratory and infectious disease research including primary lung cells, respiratory viruses and bacteria, and 5) advice, consultation and training on various aspects of molecular biology and developing or adopting new methods as needed.  


  • People

    Core Director 

    Lin Liu, Ph.D, CVHS, OSUdr. lin liu
    Regents Professor of Physiological Sciences
    Lundberg-Kienlen Endowed Chair in Biomedical Research
    Director, Oklahoma Center for Respiratory and Infectious Diseases
    Center for Veterinary Health Sciences
    Oklahoma State University-Stillwater 

    Contact Information:
    E-mail: lin.liu@okstate.edu
    Phone: (405) 744-4526
    Office: 210 McElroy Hall, Stillwater, OK 74078
    Biosketch: Dr. Liu

    Core Personnel

    Chaoqun Huang, M.D., M.S., Ph.D.chaoqun huang
    Associate Director and Assistant Research Professor
    Office: Rm 209A, McElroy Hall
    Email: chaoqh@okstate.edu

     

     

     

     

     

     

     

    yurong liangYurong Liang, Ph.D.
    Core Manager
    Office: Rm 206 McElroy Hall
    E-mail: yurong.liang@okstate.edu
    Phone: 405-744-1686

     

     

  • Standard Operating Protocol 
    MBC: Standard Operating Protocol (SOP)
    • Initiation of a project by PI
    • Consultation meeting
      • Experimental design
      • Number of samples
      • Timelines etc.
    • Assigned to MBC personnel
    • A report to PI
    • A meeting for result interpretation and problem solving.
  • Services
    Services provided by the Molecular Biology Core

    Functional studies
    • Loss-of-function: RNAi and CRISPR/Cas9
    • Gain-of-function: Transgene  overexpression
    • Mutagenesis
    • Adenovirus, lentivirus or non-virus plasmid  for gene transfer.

    Genome-wide profiling (mRNA, microRNA and lncRNA)

    • DNA microarray
    • RNA sequencing

    Assays

    • Promoter reporter assay
    • Pathway reporter assay
    • Flu A virus reporter assay
    • Real-time PCR

    Reagents

      • Respiratory viruses and bacteria
      • Cell lines and primary lung cells
      • COVID-19 resources
        • Cell models:  HEK293T-hACE2, A549-hACE2, and Vero E6
        • Human lung tissue model: Lung organoids
        • Viruses: SARS-CoV-2 virus, SARS-CoV-2 pseudovirus, adenovirus expressing human ACE2 (for creating COVID-19 mouse model) and more.

    Develop or adopt new methods
    Consultation

     

  • Equipment 
    bio-rad qx200 droplet digital pcr system

    QX200 Droplet Digital PCR System

    The QX200 Droplet Digital PCR (ddPCR) System provides absolute quantification of target DNA or RNA molecules for EvaGreen or probe-based digital PCR applications.

    nikon Ti2-e moterized micorscope

    Nikon Ti2-E Motorized microscope

    The ECLIPSE Ti2 inverted microscope delivers an unparalleled 25mm field of view (FOV) that revolutionizes the way you see. With this incredible FOV, the Ti2 maximizes the sensor area of large-format CMOS cameras without making compromises, and significantly improves data throughput.

    Nanosite N S300

    Nanosight NS300

    This versatile instrument offers the major benefit of minimum user set-up,  enhanced concentration measurements via advanced algorithms and fluorescence detection capability providing truly holistic analysis. 

    thermal cycler

    Thermal Cycler

    Common features of Bio-Rad's thermal cyclers include Peltier effect technology for precise temperature control, and thermal gradient to easily optimize PCR assays in a single run. 

    real time pcr

    Real-Time PCR

    CFX Real-Time PCR Detection Systems are powerful and flexible instruments, featuring 2–5 color multiplexing, advanced optical technology, and precise temperature control with thermal gradients. 

    nanodrop

    Nanodrop

    This compact instrument delivers full spectrum UV-Vis analysis and is ideal for rapid nucleic acid and protein quantification. 

    incu-shaker

    Incu-ShakerTM

    This instrument's outstanding temperature accuracy and uniformity are supported by proprietary heat distribution technology that ensures that temperatures are precisely maintained throughout the chamber. 

    microarray scanner

    Microarray Scanner 

    The instrument has three lasers (488/514nm, 543nm, 633nm) for maximum flexibility and is capable of scanning resolutions of 100mm to 5mm.

    amaxa nucleofector system

    Amaxa Nucleofector System

    Nucleofector® Technology offers transfection with high efficiency, low cell mortality and no detrimental physiological effect upon cells,the unique pulses deliver substrate not only to the cytoplasm but also directly to the nucleus. 

     fluostar optima microplate reader bmg biotech

    Fluostar Optima Microplate Reader (BMG Biotech)

    By integrating a high intensity xenon flash lamp with sophisticated light guide technology the FLUOstar OPTIMA covers the full wavelength range 230 to 900 nm for both excitation and emission. The FLUOstar OPTIMA can read all plate formats from 6- up to 384-well plates in all four measurement modes.

     

  • Forms
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