Immunopathology Core
About
The Immunopathology Core (IPC) provides state-of-the-art diagnostic pathology and immunology services to COBRE and other OCRID investigators. Biomedical researchers are often limited in performing complex immunopathologic analyses due to a lack of available technology/instrumentation, or by inexperience in delineating complex mechanisms associated with immune dysfunction and disease pathogenesis. These limitations highlight a critical need for a centralized resource by which investigators can receive collaborative and/or technical support necessary for their project’s success.
The IPC provides (1) access to cutting-edge assays for immunological data curation, (2) tissue processing for histopathology and downstream immunologic assays, and (3) expert support in analyses of immunopathologic data and gross/histologic lesion interpretation.
COBRE funds in Phases I-II focused on establishing, expanding, and upgrading the IPC equipment and services available to researchers. Substantial growth in Phase II highlighted the critical role of the IPC in the OCRID research infrastructure. In July 2023, the IPC was awarded Phase III funding and will focus on expanding the IPC into a self-sustaining entity able to support current and long-term state-of-the art research projects at local and regional institutions.
OCRID Core Facilities User Satisfaction Survey
OCRID Core facilities are committed to providing top quality services and technical expertise in respiratory and infectious disease for center and non-center investigators. We encourage all users to fill out a simple, ten question survey. Your responses, comments, and suggestions can help us improve our services.
- People
Core Director:
Co-Director:
Dr. Alexandra Ford, DVM, DACVP
Core Advisor:
Dr. Jerry Ritchey, DVM, PhD
Laboratory Manager:
Ms. Shannon Cowan
Histotechnologist
Mr. Caden Miller
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Contact Information
The Immunopathology Core
171 McElroy Hall
Stillwater, 74078
Phone: 405-744-4749
Email: immunopathologycore@okstate.edu
Reach out to us! Our staff and directors are always eager to discuss how we can best facilitate your research needs.
- Services
The Immunopathology Core provides immunology and pathology support including, but not limited to:
Pathology support
- Study design
- Gross necropsy, evaluation, and tissue collection
- Tissue processing and paraffin embedding of tissue sections
- Routine (H&E) and special histologic staining
- Gross and histopathological evaluation and interpretation of necropsy tissues
- Photomicroscopy, image analysis, digital scanning
- Lesions scoring and interpretation of pathologic findings
- Immunohistochemistry
- Slide Scanning
- Photomicrography
Immunology support
- Fluorescence-activated cell sorting BD FACSAria Special Order Research Product (SORP) performs high speed sorts
- Bio-Rad Bio-Plex 200 System
- ELISA plate washer
- Immunofluorescence
- Technical support for evaluations using confocal microscopy and interpretation of images obtained by electron microscopy
- Support for basic tests of immune function, antigen-specific T cell assays, and immune cell activation
We are here to help!!!
Immunopathology Core Citation and Acknowledgement Guidelines
Acknowledgement of the core in submitted manuscripts is critical for the IPC's continued success. Please use the following statement in any publication where the IPC facilities, services, and/or personnel were used:
"The authors acknowledge resources and support from the Immunopathology Core, part of the Oklahoma State University's Oklahoma Center for Respiratory and Infectious Diseases (OCRID)."
- Equipment
Equipment
Immunology and Characterization of Immune Response
- Biosafety cabinet
- Refrigerated microfuge
- Table-top refrigerated centrifuge
- ELISA plate washer
- CO2 incubators and equipment for cell culture
- Flow cytometry and cell sorting
- Dedicated Laboratory computers/printers/IT support
Flow cytometer
- BD FACSAria SORP has 4-lasers (violet, blue, yellow-green, red) and is capable of aseptic, high-speed sorts. 2-way and 4-way sorts can be received into a variety of tubes including 1.5 ml Eppendorf tubes, 12x75mm tubes, or 15 ml conical tubes. The automated cell deposition unit (ACDU) allows sorting into multiwell plates and onto microscope slides. The cytometer is housed in a Baker BioProtect IV biosafety cabinet to allow sorting of BSL2 infected cells or pathogens.
Please consider overlap in your panel when choosing fluors
For assistance in designing a panel please contact Ms. Shannon Cowan or refer to this site: https://fluorofinder.com/
Bio-Plex 200 Array Reader
The Bio-Plex 200 system is a suspension array system based on the xMap technology by Luminex. Bio-Plex offers protein and nucleic acid researchers a reliable multiplex assay solution that permits analysis of up to 100 biomolecules in a single sample. Multiple manufacturers produce kits that are compatible with the Bio-Plex. Some of these are listed below.
- Forms
- Price Information
Immunology Services
COBRE
Aria with Operator
$60/hour
Aria Self-serve
$30/hour
Sorting
$80/hour
ELISA Washer
$20/plate
ELISA Reader
FREE
Cytometry - Data Analysis
$30/hour
Bio-Plex Immunoassay
$40/plate
Technical support including training, consult, other
$15/half hour
Histology Services
Gross necropsy eval including tissue collection
$120/animal
Histopathologic eval including H&E slides
$120/animal
Tissue trimming
$5/block
Process and embed only
$8/block
H&E slides
$12/slide
Slide preparation (no interp.)
$12/slide
Research decalcification
$12/block
Serial H&E stained slides
$7/slide
Research IHC^
$21-41/slide
Serial unstained slides
$7/slide
Large slide box
$12/box
Small slide box
$7/box
Technical support including training, consult, other
$15/half hour